his project aims to leverage Advanced Physics Refinement (APR) to create a high-throughput mechano-phenotyping technology by establishing a digital twin of a microfluidic device and quantifying cellular behavior in complex 3D geometries with realistic red blood cell (RBC) backgrounds. The first goal is to develop a digital twin of the entire microfluidic device that can be coupled with in vitro devices to enable high-throughput mechano-phenotyping. The second goal is to create a protocol for quantifying likely cellular behavior across the full ensemble of potential RBC configurations in whole blood measures.